Ammonium formate, methanol, phosphoric acid, sodium dodecyl sulfate (SDS) and sodium hydroxide were acquired from Fisher Scientific (Fair Lawn, NJ). Cotinine was purchased from Alfa Aesar (Ward Hill, MA) and a banal band-aid (1 mg/mL) was able in booze and stored at −20°C. The micellar accomplishments electrolyte (BGE) independent 50 mM phosphoric acerbic and 75 mM SDS (pH 2.5). The non-micellar BGE was 50 mM phosphoric absorber (pH 2.5). The high-conductivity absorber (HCB) was 75 mM phosphoric absorber (pH 2.5). The sample cast band-aid for CE assay was 0.1 mM phosphoric acid. Purified baptize from a Millipore Nanopure baptize arrangement (Barnstead, Dubuque, IA) was acclimated to adapt all solutions.
The procedures for advertisement mice to SHS accept been appear previously23. Briefly, macho C57BL/6 mice with ages of 8 weeks were purchased from the Jackson Laboratory (Bar Harbor, ME). All abstracts were performed in accordance with the allegorical attempt of the Guide for the Care and Use of Laboratory Animals and accustomed by the Institutional Animal Care and Use Committee (IACUC) of the Georgia Regents University. A whole-body smoke assimilation arrangement (Scireq Inc., Phoenix, AZ) was acclimated for tobacco smoke exposure. Research cigarettes 3R4F (University of Kentucky) were smoked at 1 puff/min and 10 puffs/cigarette. The mice were apparent to ancillary beck tobacco smoke at a agenda of
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